appD [2018-02-13T09:02:32.000Z]

oligopeptide ABC transporter (ATP-binding protein)

Locus: BSU11360

Isoelectric point: 5.39

Molecular weight: 36.16 kDa

Protein length: 328 aa

Gene length: 984 bp

Function: uptake of oligopeptides

Product: oligopeptide ABC transporter (ATP-binding protein))

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of peptides
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of peptides
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,211,477 → 1,212,463

The protein

Protein family

• ABC transporter domain (according to Swiss-Prot)

Paralogous protein(s)

• DppD, OppD

Structure

• 4FWI (from Caldanaerobacter subterraneus, 38% identity) PubMed

Localization

• attached to the cell membrane (via AppB-AppC) PubMed

Expression and Regulation

Operons

• Genes: appD-appF-appA/1-appA/2-appB-appC
  Description: PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon
  • CodY: repression, PubMed PubMed, in CodY regulon
  • ScoC: repression, PubMed, in ScoC regulon

  Regulation

  • repressed by CodY PubMed

Biological materials

Mutant

• GP2100 (D(appD-appF-appA/2-appB-appC)::spc), available in Jörg Stülke's lab
• BKE11360 (ΔappD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTAGCTCCCCTTTCCC, downstream forward: _UP4_GAGGAAGAGGGTGCCGAACA
• BKK11360 (ΔappD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTAGCTCCCCTTTCCC, downstream forward: _UP4_GAGGAAGAGGGTGCCGAACA

References

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